Composite

Part:BBa_K4129005:Design

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-02)


mCherry cassette under the regulation of the promoter of the Saccharomyces cerevisiae FDH2 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
    Illegal BglII site found at 1915
    Illegal BamHI site found at 2175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3046009 is designed to allow for its promoter to be switch using Golden Gate assembly. BsaI sites were added at the estremities of K4129001 by PCR to allow for a one step Golden Gate reaction.



Source

The part was obtained by inserting the scFDH2 promoter (BBa_K4129001) in place of the prokaryotic promoter of BBa_K3046009 by Golden Gate assembly.

References